Explain the procedure of ELISA.
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Explanation:
An enzyme-linked immunosorbent assay (ELISA) is used to detect the presence of an antigen in a sample. The antigen is immobilized to the well of a plate by adsorption, or captured with a bound, antigen-specific antibody. A detection antibody is then added forming a complex with the antigen, if present.
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Answer:
1) Bind Samples To Support, 2) Add Substrate, 3) Add Primary Antibody And Wash, 4) Add Secondary Antibody-enzyme Conjugate And Wash B.
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