Explain the process of gel electrophoresis technique?
Answers
Explanation:
Gel electrophoresis is a technique used to separate DNA fragments according to their size.
DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel.
DNA fragments are negatively charged, so they move towards the positive electrode.
Answer:
Gel-electrophoresis is an analytical separation procedure used in molecular biology to separate and identify molecules such as DNA, RNA, protein, complexes on the basis of size.
The separation is achieved by loading the sample in a gel made up of small pores and setting an electric field across the gel. The molecules migrate across the gel based on the electric charge and smaller molecule move faster than the larger molecule.
A size separation is received. THE gel offers molecular sieving effect and thus helps in separation.