Explain the process of gram staining
Answers
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Gram stain: ... Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they don't rinse out during the staining procedure. The addition of iodide, which binds to crystal violet and traps it in the cell. Rapid decolorization with ethanol or acetone.
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Answer:
This method is used to identify bacterial species into two communities i.e. Gram positive and gram negative. This method is based on chemical and physical properties of their cell walls. It can be used to detect peptidoglycan, which is present in a thick layer in Gram positive bacteria. Purple/blue colour refers to the gram positive bacteria. Red colour stain refers to the gram negative bacteria. This method is very popularly used in the identification of bacterial organism.