Question

explain the process of nicking and re-annealing??

Answer 1

A nicking enzyme (or nickingendonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved.


Annealing is a heat treatment process which alters the microstructure of a material to change its mechanical or electrical properties. Typically, in steels, annealing is used to reduce hardness, increase ductility and help eliminate internal stresses.

Answer 2

A nicking enzyme (or nickingendonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved.