Explain the process of observation of nucleus incheek cell in the laboratory?
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To Prepare Stained Temporary Mount of Human Cheek Cells
Materials Required
in the image
Real Lab Procedure
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.Place the cells on a glass slide that has water on it.Mix the water and the cheek cells using a needle and spread them.Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.Take a few drops of glycerine using a dropper and add this to the test mixture.Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.Using a brush and needle, press the cover slip gently to spread the epithelial cells.Remove any extra liquid around the cover slip using a blotting paper.Place this glass side on the stage of the compound microscope and view it.
Observations
A large number of flat and irregular-shaped cells are observed.The cells do not have a cell wall. However, each cell has a thin cell membrane.A deeply stained nucleus is observed at the centre of each cell.No prominent vacuoles are observed in the cells.
Conclusion
As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.
Simulator Procedure (as performed through the Online Labs)
The ‘Select view’ drop down list allows you to select either the Microscope or Binocular View (It is the 'Binocular view' through which you can see the cell structure as viewed through the microscope).To select the sample, you can use the ‘Select sample’ drop down list.To change the power of the lens, you can choose a lens from the ‘Select objective lens’ drop down list.For coarse adjustments, you can either click on the up and down arrow of ‘Coarse adjustment’ knob, or click on the left and right arrows of 'Course Adjustment' seen on the left controls panel.For fine adjustments, you can click on the left and right arrows of ‘Fine adjustment’ seen on the left controls panel.You can redo the experiment by clicking on the ‘Reset’ button.
Precautions
Ensure toothpick used to scrape the cheek is clean, so it does not cause infection to the cheek.Extra glycerine stain should be removed using blotting paper.
Materials Required
in the image
Real Lab Procedure
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.Place the cells on a glass slide that has water on it.Mix the water and the cheek cells using a needle and spread them.Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.Take a few drops of glycerine using a dropper and add this to the test mixture.Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.Using a brush and needle, press the cover slip gently to spread the epithelial cells.Remove any extra liquid around the cover slip using a blotting paper.Place this glass side on the stage of the compound microscope and view it.
Observations
A large number of flat and irregular-shaped cells are observed.The cells do not have a cell wall. However, each cell has a thin cell membrane.A deeply stained nucleus is observed at the centre of each cell.No prominent vacuoles are observed in the cells.
Conclusion
As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.
Simulator Procedure (as performed through the Online Labs)
The ‘Select view’ drop down list allows you to select either the Microscope or Binocular View (It is the 'Binocular view' through which you can see the cell structure as viewed through the microscope).To select the sample, you can use the ‘Select sample’ drop down list.To change the power of the lens, you can choose a lens from the ‘Select objective lens’ drop down list.For coarse adjustments, you can either click on the up and down arrow of ‘Coarse adjustment’ knob, or click on the left and right arrows of 'Course Adjustment' seen on the left controls panel.For fine adjustments, you can click on the left and right arrows of ‘Fine adjustment’ seen on the left controls panel.You can redo the experiment by clicking on the ‘Reset’ button.
Precautions
Ensure toothpick used to scrape the cheek is clean, so it does not cause infection to the cheek.Extra glycerine stain should be removed using blotting paper.
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