Explain the process of staining of bacteria introduced by Hans Christian Gram in 1884 and explain what are Gram positive bacteria and Gram Negative bacteria? Well explained answer will be marked as the brainliest.
Answers
Answer:
the gram staining technique is used to classify the bacteria on the bases of their different cell wall constituent of the bacteria. The gram stain procedure distinguish between Gram positive and Gram negative considering on the cell colouration as red or violet.
Gram positive bacteria stain violet due to the presence of thick layer peptidoglycan in their cell wall.
Gram negative bacteria stain red due to the presence of thinner peptidoglycan.
Explanation:
HOW THE STAINING PROCESS OCCUR
It involves three step
- staining with a water soluble dye
- decolorization
- counterstaining with sefanin
Based on the difference in the thickness of the peptidoglycan layer in the cell membrane .
The Gram positive bacteria retain the violet colour even after the decolorization process.
The Gram negative bacteria lose the colour as it has thinner peptidoglycan layer of cell membrane as crystal violet stain is dissolved in the process of decolorozation process and are stained with sefanine in the last step