Explain the rationale of each the test reagent used. (Benedict's test, Barfoed's test, Seliwanoff's test)
Answers
Benedict's test would determine if the sample is a reducing sugar, and Barfoed's test would determine if it is a monosaccharide or disaccharide.
Benedict's solution can be used to test for the presence of glucose in urine. Some sugars such as glucose are called reducing sugars because they are capable of transferring hydrogens (electrons) to other compounds, a process called reduction.
positive test with Benedict's reagent is shown by a color change from clear blue to brick-red with a precipitate. Generally, Benedict's test detects the presence of aldehydes, alpha-hydroxy-ketones, and hemiacetals, including those that occur in certain ketoses.
Explanation:
BARFOED'S TEST: barford's test is a chemical test used for detecting the presence of monosaccharides it is based on the reduction of copper