Question

explain the role of plasmid in recombinant dna technology

Answer 1

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⭐Two enzymes are used to produce recombinant plasmids.
Restriction enzymes cut DNA at specific 4- to 8-bp sequences, often leaving self-complementary single-stranded tails (sticky ends).
These enzymes are used to cut long DNA molecules into multiple restriction fragments and to cut a plasmid vector at a single site.

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Answer 2

H0LA..

A plasmid is an extra chromosomal genetic material found in bacteria,usually they are circular
In rDNA tech. Plasmid is used as a vector
A vector is defined as Molecular vehicle which transfer desirable gene into the host cell
Note that, all vectors are not plasmids, for eg- YAC (yeast artificial chromosome ) and BAC ( bacterial artificial chromosomes are vectors but not plasmids)

As plasmid have OriC ( origin of replication ) and Mcs ( multiple cloning site) , it is considered as a vector in rDNA Tech.

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