Explain the step-by-step process of making a temporary slide of human cheek cell ?
Answers
Answered by
1
Requirements
Sterile cotton swab
Clean, sterile microscope slides
Microscope cover slips
Methylene Blue solution (0.5% to 1%)
A dropper
Blotting paper/Tissue paper
Microscope
How to Prepare a Wet Mount of Cheek Cells
Before starting, it is always important to ensure that the working surface is clean and that you are wearing a pair of clean gloves to avoid contamination.
Cheek cells can be easily obtained by gently scraping the inside of the mouth using a clean, sterile cotton swab.
Once the cells have been obtained, the following procedure is used for cheek cell wet mount preparation;
place a drop of physiological saline on a clean microscopic slide (central part of the slide)
smear the cotton swab on to the center (part containing the saline drop) of the clean slide for about 4 seconds to get the cells on to the center of the slide
add a drop of methylene blue solution on to the smear and gently place a cover slip on top (to cover the stain and the cells)
any excess solution can be removed by touching one side of the slide with a paper towel or blotting paper.
place the slide on the microscope for observation using 4 x or 10 x objective to find the cells
once the cells have been found, they can then be viewed at higher magnificatio
*Note- Used cotton swabs and cotton towel should be safely discarded in the trash and not left lying on the working table.
Sterile cotton swab
Clean, sterile microscope slides
Microscope cover slips
Methylene Blue solution (0.5% to 1%)
A dropper
Blotting paper/Tissue paper
Microscope
How to Prepare a Wet Mount of Cheek Cells
Before starting, it is always important to ensure that the working surface is clean and that you are wearing a pair of clean gloves to avoid contamination.
Cheek cells can be easily obtained by gently scraping the inside of the mouth using a clean, sterile cotton swab.
Once the cells have been obtained, the following procedure is used for cheek cell wet mount preparation;
place a drop of physiological saline on a clean microscopic slide (central part of the slide)
smear the cotton swab on to the center (part containing the saline drop) of the clean slide for about 4 seconds to get the cells on to the center of the slide
add a drop of methylene blue solution on to the smear and gently place a cover slip on top (to cover the stain and the cells)
any excess solution can be removed by touching one side of the slide with a paper towel or blotting paper.
place the slide on the microscope for observation using 4 x or 10 x objective to find the cells
once the cells have been found, they can then be viewed at higher magnificatio
*Note- Used cotton swabs and cotton towel should be safely discarded in the trash and not left lying on the working table.
Similar questions