Question

Explain the steps involved in the production of genetically engineered insulin

Answer 1

Ist prepared 2 DNA sequences corresponding to chain A and B of human insulin. It was introduced in plasmid of Escherichia coli to produce insulin chains .Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin or humulin.Eli Lilly and American company made this humulin.

Answer 2

Human Insulin production steps are:-

1. Isolation of Donour or DNA segment
2. Formation of Recombinant DNA
3. Production of Multiple copies of rDNA.
4. Introduction of rDNA in recipient organisms.
5. Screening of Transformed cell.