Question

Explain the synthesis of double stranded complementary DNA from mRNA .​

Answer 1

Answer:

(1) Messenger RNA is incubated with reverse transcriptase, which uses the mRNA as a template for synthesis of a complementary DNA (cDNA) strand. ... (3) DNA polymerase can now synthesize the complementary DNA strand. The looped-around 3prime end of the first DNA strand can often be used as a primer.

Answer 2

Answer:

In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes.

Explanation:

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