Question

Explain the term Denaturation of DNA.

Answer 1

Answer:

DNA denaturation deals with the loss of three dimensional structure of DNA

Explanation:

DNA act as a repository of genetic information of our body.it contain specific 3 dimensional structure in which the complementary base pair are interract with each other by hydrogen bonding.

          DNA denaturation hampers the stability of DNA structure by breaks the two antiparrallely arranged polynucleotide strands.This ultimately result in the loss proper functioning of DNA.There are many agents that denature DNA

1 Heat High temperature breaks the hydrogen bonds present between complementary base pair thus affecting the stability of DNA .

2 PH  high  PH or low H+ ion concentration result in the loss of hydrogen bonding between the the complementary base pair thus affecting the structure of DNA.

3  Chemical agents such as acetone,alcohol,urea contain specific functional group that can form hydrogen bond with electronegative part of the nitrogen bases

4 Salt concentration High salt concentration affect the negative charge of the sugar phosphate back bone from interraction with one another there dis stabilizing the DNA structure .

Answer 2

DNA denaturation is the process of generating the two single strands of DNA by melting the double strands.

Explanation:

• The process of denaturation of DNA involves the breaking of the hydrogen bond between the bases.
• The protein loses its shape causing the denaturation of DNA; the main reason for protein losing its shape is due to external stress and the exposure to acid and base, and heat.
• The denaturated DNA can reformulate the hydrogen bond which is called as renaturation.