.Explain the test for protein with diagram.
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Answer:
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Tests to conform to the presence of Protein-
Aim-
To perform a simple test to identify the presence of proteins in the given plant and animals food sample.
Materials Required-
1) Stirrer
2)Dropper
3)Water bath
4) Distilled water
5)Bunsen burner
6) Test tube holder
7)Clean and dried test tubes
8) Food sample – Milk, Apple, Paneer, Butter, Cheese, etc.
Chemical Required-
1) Nitric acid
2) Sulfuric acid
3) Sodium nitrite
4) Mercuric sulfate
5) Pyridine solution
6) Ninhydrin reagent
7) Sodium hydroxide
8) Copper sulfate solution
9) Biuret reagent solution or solution of copper sulfate and caustic soda.
A) Procedure- Biuret Test
1) Take a cleaned and dried test tube.
2) Add the food samples of your choice into the test tubes.
3) Add 2ml of sodium hydroxide and 5 to 6 drops of copper sulfate solution to it.
4) Shake the test tube gently to mix the ingredients thoroughly and allow the mixture to stand for 4 – 5 minutes.
5) If there is the appearance of bluish- violet color, it indicates the presence of protein.
B) Procedure-Xanthoproteic Test
1) Take a cleaned and dried test tube.
2) Add the food samples of your choice into the test tubes.
3) Add a few drops of concentrated sulfuric acid and shake the test tube.
4) Heat the test tube gently on a Bunsen burner.
5) If there is a formation of yellow precipitate, then the presence of protein is confirmed.
SEE THE ATTACHMENT- # 1
C) Procedure- Millions Test
1) Take a cleaned and dried test tube.
2) Add the food samples of your choice into the test tubes.
3) Add 2-3 drops of Millon’s reagent and shake well.
4) Observe the change.
5) If there is the formation of white precipitate or if the sample changes to brick red on heating then the presence of protein is confirmed.
D) Procedure- Ninhydrin Test:
1) Take a cleaned and dried test tube.
2) Add the food samples of your choice into the test tubes.
3) Add 1-2ml of ninhydrin solution to it and shake the test tube.
4) Boil the mixture and observe the change.
5)If there is the appearance of deep blue or purple color then the presence of protein is confirmed.
RESULTS- The given food sample – Milk, Apple, Paneer, Butter, Cheese, etc contains proteins in it. The protein test is used to measure the total amount of protein present in the given blood samples. Usually, Doctors prescribe this test if a patient is suffering from any of the food deficiency diseases or other disorders like fatigue, weight loss, kidney or liver disease, etc.
Precautions- Be careful while handling chemicals and other acidic solutions.
2) Refer to the lab manual before beginning with the experiment.
3) Wear lab aprons and hand gloves during the experiment.
4) Use droppers to take the reagents from the bottle.
SEE THE ATTACHMENT- # 1
