Explain the types of ELISA with advantages and disadvantages.
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Elisa is a test for diagnosis of aids. it uses the interaction between the antibodies and antigen complex to check the presence of HIV .
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ELISA has a number of benefits compared to the other immunoassay techniques. It is often preferred because it has high sensitivity and specificity. ELISA also offers more accuracy compared to other techniques such as radioimmunoassay (RIA) tests. ELISA assays are usually in 96 well microplate format.
Potential high background: all proteins in the sample bind to the surface. No signal amplification. Low flexibility: the primary antibody must be conjugated
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