Question

Explain the various steps involved in the production of artificial insulin.

Answer 1

Answer:

Insulin used for diabetes was earlier extracted from pancreas of slaughtered cattle and pigs.

It consist of two short polypeptide chains- chain A and chain B that are linked together by disulphide bridges.

In mammals even in humans, insulin is synthesised as a pro-hormone also need to be processed before it becomes a fully mature and functional hormone. It contains an extra stretch called C-peptide

Explanation:

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Answer 2

Human Insulin production steps are:-

1. Isolation of Donour or DNA segment
2. Formation of Recombinant DNA
3. Production of Multiple copies of rDNA.
4. Introduction of rDNA in recipient organisms.
5. Screening of Transformed cell.