Explain the working principle and applications of inverse pcr
Answers
In the inverse PCR, the amplification of unknown flanking regions of DNA carried out using the known DNA sequence primers.
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the primers which amplify the flanking (unknown) region near the know DNA template.
Quite difficult to understand?
No problem, the present article is only about the inverse PCR. I know all other resources available online about the inverse PCR are confusing.
In this present article, we will understand the mechanism of inverse PCR and its application in molecular genetics.
I bet you read the article till the end, you will understand the fundamental basics behind the inverse PCR.
The content of the article,
What is an inverse PCR?
Principle of inverse PCR
Steps and procedure of inverse PCR
Protocol for inverse PCR
Application of inverse PCR
Limitation of inverse PCR
Conclusion
Answer:
Principle of inverse PCR: With the help of the sequence information of known DNA region, the unknown flanking region of the DNA or the inserted DNA is amplified into the cyclic enzymatic reaction using the known DNA sequence-specific primers.