Explain three essntial steps of P C R technique.
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denaturation , annealing, extension
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There are three main stages:
•Denaturing – when the double-stranded template DNA is heated to separate it into two single strands.
•Annealing – when the temperature is lowered to enable the DNA primers to attach to the template DNA.
•Extending – when the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.
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