Question

Explain tissue culture

Answer 1

$\huge{ \underline{ \overline{ \mid{ \bold \red{ANSWER - }} \mid}}}$

$\underline{ \rm \blue{Tissue \: Culture - }}$Tissue culture is a technique of propagating plants by culturing cells or tissue in a culture medium or in lab.

➡️When vegetative propagation is not possible in a plant, its bud, shoot apex or some other part is used for micropropagation. This is called explant.

$\underline{ \underline{ \rm \red{STEPS \: INVOLVED \: IN \: TISSUE \: CULTURE - }}}$

➡️A sterilised bud or shoot apex is cultured in a nutrient medium.

➡️Soon its cells grow and divide to form a mass of undifferentiated cells called callus. This can be maintained and multiplied for a very long period.

➡️When a small proportion of callus is transferred to a nutrient with specific hormones, it differentiates into miniature plants called Plantlets.

➡️After 4-6 weeks, plantlets are transferred to pots or to soil.

By this method, indefinite number of plants can be obtained from a small mass if parental tissue.

$\underline{ \underline{ \rm \pink{ADVANTAGES \: OF \: TISSUE \: CULTURE - }}}$

◼️It provides rapid propagation of identical individuals.

◼️Very small explants can be used for micropropagation.

◼️It can be carried out throughout the year and usually plantlets obtained are disease free.

◼️Plants can be grown even from sterile hybrids.

◼️Only a small space is required for tissue culture.

✏️There is a disadvantage of tissue culture. The survival rate is low of these plants.

Answer 2

Answer:

The procedure in which a small tissue pieces or organs is removed from a donor plant and cultured aseptically on a nutrient medium is called Tissue Culture. This production is also known as micro propagation technique.