Explain what is the technique used to detect protein concentration?
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The simplest and most direct assay method for protein concentration determination in solution is to measure the absorbance at 280 nm (UV range). Amino acids containing aromatic side chains (i.e., tyrosine, tryptophan and phenylalanine) exhibit strong UV-light absorption
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To detect protein or other bio-molecules, mass spectrophotometry is used. It works on the principle of light absorption method. This instrument measures the amount of light of a specified wavelength. Moreover, the amount of light absorbed by the medium is directly proportional to the concentration of the bio-molecules or protein.
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