Question

Explain why it is not possible to see both the palisade cells and the stage micrometer scale at the
same time.​

Answer 1

Answer:

− 10 = 51 divisions on the eyepiece graticule are equivalent to 250 μm on the stage micrometer. One division on the eyepiece graticule is equivalent to μ 250 51 μ m on the stage micrometer: = 4.9 μm (to two significant figures ).

Answer 2

It is not possible to see both the palisade cells and the stage micrometre scale at the same time because it will not show different calibration power.

• A stage Micrometer scale is generally a microscope slide that consist of a finely divided scale on it present on the surface. The scale is used for a good and known true length. With the help of patterns of eyepiece graticule, the scale is used for calibration of optical systems.
• Each lens of the microscope must be separately calibrated during calibration beginning with the lowest power objective.
• The stage micrometre scale and the palisade cells are observed at different times to increase the chances of having different calibration power for both of them.

Hence, To view the distinct power of calibration, both the stage micrometer scale and the palisade cells are observed under different duration.