Question

Explain why Proline is seen as an exception when a ninhydrin complex is formed.

Answer 1

Answer:

An exception is the amino acid proline or hydroxyproline , which as secondary amino acids have a secondary (NH) amino group instead of a primary (NH 2 ) . ... When reacting with a primary amino acid, which has an NH 2 group, has an absorption maximum of 570 nm, and reacts to form a violet dye called Ruhemann's purple

Answer 2

Answer:

In these amino acids, the N is free to react with ninhydrin. However, in proline, the N is not available for reaction as it is locked in the ring structure. Therefore no ammonia is produced, so no blue color is presented

Explanation:

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