Question

Explain why staining and use of a sharp cutting blade are important during the preparations of temporary slides

Answer 1

Answer: hope it helps

Explanation:

To make the specimen to be distinct

ii)To obtain thin sections

iii)To allow light to penetrate through the specimen

iv)To maintain the cells of the specimen turgid.

Answer 2

Answer:

The importance of staining during the preparations of temporary slides -

Staining helps to visualize the cells under microscopes, and some stains even penetrate the cell for the visualization of cell components and metabolic processes. For example, acetocarmine and hematoxylin are used to stain the nucleus.

Gram staining is also an example to visualize the bacterial cell under the microscope for the differentiation between gram-positive and gram-negative bacteria. Crystal violet and safranin are two dyes used in the preparation of temporary slides for gram staining. Gram-positive bacterial appear dark blue in color and gram-negative bacteria appear pink in color.

The importance of the use of sharp cutting blades during the preparation of temporary slides - It helps to cut the tissues into very small pieces. It also prevents the deposition of a microorganism on the tissue.

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