explain why the selection by HAT medium in the hybridoma technology is a negative selection.
Answers
Answered by
6
Hybridoma selection using HAT medium
Unfused spleen cells are easily selected against since they do not replicate in culture. Unfused myelomas can be selected against using media containing HAT. The aminopterin found in the medium blocks the de novo DNA nucleotide synthesis pathway. Only hybridomas survive.
Answered by
0
Answer:
Unfused myelomas can be selected against using media containing HAT. ... However, these myelomas are unable to do so since they are deficient in an enzyme called HGPRT, which is required for the salvage pathway. Hence, myelomas are unable to replicate in culture. Only hybridomas survive.
Similar questions
World Languages,
5 hours ago
World Languages,
5 hours ago
History,
9 hours ago
English,
9 hours ago
Math,
7 months ago
Social Sciences,
7 months ago