Biology, asked by Nazrana4722, 1 year ago

expression of selenometheonine substituted proteins in non-metheonine auxotrophic e. coli

Answers

Answered by keshrishi9898

0

The protein is expressed in any E. coli strain, with no need for methionine synthesis

deficient strains. Feed back inhibition of methionine biosynthesis is facilitated by adding

amino acids prior to induction. The following protocol is for 10l culture (version 11 June

2003)

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