extinction coefficient concentration wavelength betanin
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Spectrophotometry UV-Vis, with calibration curve (SWCC), High-performance liquid chromatography
(HPLC), and Spectrophotometry with an extinction coefficient (SWEC) were used for betanin
quantification in pulp and peel of pitaya. The concentrations of pulp betanin measured by the three analytical
methods did not differ significantly, as assessed by the Scheffe method (P > 0.05). The mean of pulp betanin was 2.092 mg/g dry
sample. With respect to the peel samples, there was a significant difference: a lower betanin concentration was obtained by HPLC.
Since the SWCC method required a standard solution, and that HPLC was time-consuming, the SWEC method represented the best
choice for betanin quantification in pulp in order to reduce cost, time, and wastes.
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