Fats are tested by:
1
Benedict's and Fehling's tests
2
Lugol's iodine solution
3
Millon's test
4
Sudan -III dye test
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Fats are tested by Sudan III test.
Detection of presence of fats (lipids) : Sudan III test :-
• Take 2mL of mustard oil in a test tube.
• Add equal volume of water to it and shake.
• Add a pinch of Sudan III and shake again.
• As the layers separate out, the lipid layer is seen to be stained red, whereas water layer remains uncoloured. This indicates the presence of lipids.
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