First successful pollen culture was done by
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The first successful pollen culture was done by Guha and Maheshwari in 1964.
- Pollen culture can also be referred as microspore culture.
- It can be defined as a technique in which haploid plants are obtained from isolated pollen grains.
- In this culture, the immature pollen is used as the explant to get the haploid plants directly.
- For this purpose, the flower buds are collected, surface sterilized and then the anther lobes are dissected out from the flower buds.
- With the use of pollen culture, the sequence of androgenesis can be observed starting from a single cell.
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