Question

Following is the product produced in
phosphatase positive test which gives
yellow color when milk sample is added
in reaction mixture.​

Answer 1

Answer:

Following are the reagent of this test

Sodium Carbonate-Bicarbonate Buffer - Dissolve 3.5 g of anhydrous sodium carbonate and 1.5 g of sodium bicarbonate in one litre of distilled water.

Buffer Substrate - Dissolve 1.5 g of disodium p-nitrophenyl phosphate in one litre of sodium carbonate-bicarbonate buffer. This solution is stable if stored in a refrigerator at 4°C or less for one month but a colour control test should be carried out on such stored solutions