For a plasmid to be cloning vector the minimum numbers of element required are
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tools for recombination are required for plasmid to be cloning
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a. An origin of replication
b. an antibiotic resistance marker
c. a restriction site
Explanation:
For a plasmid to be cloning vector the minimum numbers of element required are
a. An origin of replication
b. an antibiotic resistance marker
c. a restriction site
- A small segment of DNA into which a desired foreign DNA fragment can be integrated for producing more copies of the desired DNA fragment is called as cloning vector.
- Cloning vectors play a major role in gene cloning process.
- Cloning vectors contain the following:
- Origin of replication
- Selectable marker gene (Eg: Antibiotic resistance marker)
- Unique restriction site
So, An origin of replication, antibiotic resistance marker and a restriction site is required
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