Question

For detecting proteins a suitable reagent in the following is
A. Iodine solution
B. Bromine water
C. Alkaline copper sulfate solution
D. A piece of paper

Answer 1

The answer is C. Alkaline copper sulfate solution.

Proteins are composed of peptide bonds (in their primary structure).

When copper sulfate in alkaline condition reacts with the peptide bond of proteins, it produces a purple color change.

The test used for detecting proteins is called the Biuret test.

Primarily, when conducting the Biuret test, the protein solution is heated to allow the peptide bonds to be exposed.

Answer 2

A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.

C. Alkaline copper sulfate solution

is the right option