Question

For induction of alien dna in host cell

Answer 1

The induction of a foreign DNA into a host cell can be done in the following ways:

1. Using vectors:

Viral transduction- insertion of foreign DNA using virus as a vector

Transformation( in case of bacteria),

Transfection( in case of eukaryotic cells)

Using Agrobacterium( Ti plasmid)

2. Microinjection

3.Liposomal method( inserting using lysosomes)

4.Electroporation

5.Biolistics or gene gun technique

6.Laser method

7.Fiber mediated method

8.Heat shock method

Answer 2

When the DNA is introduced in the tissue culture cells with direct microinjection and nuclei of the cells then glass micropipette are drawn out to an extremely thin diameter.

Procedure requires the fairly sophisticated equipments that is highly suitable for the puller to make the needles as well as micromanipulator to position needles correctly. Injected DNA would integrate at random into nuclear DNA.