For metal link immobilization the material
used is
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Titanium
Calcium
(0)
Boron
Sulphur
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Traditionally, four methods are used for enzyme immobilization, namely (1) non-covalent adsorption and deposition, (2) physical entrapment, (3) covalent attachment, and (4) bio-conjugation (Fig. 2). Support binding can be physical or chemical, involving weak or covalent bonds.
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