Question

For the sepration of closely related compounds coloum used are.

Answer 1

${\tt{\pink{\bold{\huge{ᴀɴsᴡᴇʀ}}}}}$

ᴛʜᴇ ᴜsᴇ ᴏғ ғʟᴜᴏʀɪɴᴀᴛᴇᴅ sᴛᴀᴛɪᴏɴᴀʀʏ ᴘʜᴀsᴇs ɪɴ ʟɪǫᴜɪᴅ ᴄʜʀᴏᴍᴀᴛᴏɢʀᴀᴘʜʏ ᴀɴᴅ ʜʏᴘʜᴇɴᴀᴛᴇᴅ ᴛᴇᴄʜɴɪǫᴜᴇs ʜᴀs ʙᴇᴄᴏᴍᴇ sɪɢɴɪғɪᴄᴀɴᴛ ɪɴ ʀᴇᴄᴇɴᴛ ʏᴇᴀʀs. ᴇᴀʀʟʏ ᴀᴘᴘʟɪᴄᴀᴛɪᴏɴs ɪɴ ᴛʜᴇ ᴇғғᴇᴄᴛɪᴠᴇ sᴇᴘᴀʀᴀᴛɪᴏɴ ᴏғ ᴘᴀᴄʟɪᴛᴀxᴇʟ ᴘʀᴏᴠɪᴅᴇᴅ sᴜᴄʜ ᴘʜᴀsᴇs ᴍᴜᴄʜ ɴᴏᴛᴏʀɪᴇᴛʏ; ʜᴏᴡᴇᴠᴇʀ, ᴍᴏʀᴇ ʀᴇᴄᴇɴᴛ sᴛᴜᴅɪᴇs ғᴏᴄᴜsɪɴɢ ᴏɴ ᴏʀᴛʜᴏɢᴏɴᴀʟɪᴛʏ ᴛᴏ ᴛʀᴀᴅɪᴛɪᴏɴᴀʟ ᴀʟᴋʏʟ ᴘʜᴀsᴇs ʜᴀs ɪɴᴠɪᴛᴇᴅ ᴇᴠᴇɴ ʙʀᴏᴀᴅᴇʀ ᴀᴛᴛᴇɴᴛɪᴏɴ. ᴅᴜᴇ ᴛᴏ ᴛʜᴇ ᴅɪғғᴇʀᴇɴᴛ ʀᴇᴛᴇɴᴛɪᴏɴ ᴍᴇᴄʜᴀɴɪsᴍs ғʟᴜᴏʀɪɴᴀᴛᴇᴅ sᴛᴀᴛɪᴏɴᴀʀʏ ᴘʜᴀsᴇs ᴘʀᴏᴠɪᴅᴇ, ᴛʜᴇʏ ᴀʀᴇ ᴏғᴛᴇɴ ᴇᴍᴘʟᴏʏᴇᴅ ғᴏʀ ᴛʜᴇ sᴇᴘᴀʀᴀᴛɪᴏɴs ɴᴏᴛ ᴇᴀsɪʟʏ ᴏʙᴛᴀɪɴᴇᴅ ᴜsɪɴɢ ᴄᴏᴍᴍᴏɴ ᴄ ᴘʜᴀsᴇs. ᴀᴘᴘʟɪᴄᴀᴛɪᴏɴs ɪɴ ᴀʀᴇɴᴀs sᴜᴄʜ ᴀs ʙɪᴏᴘʜᴀʀᴍᴀᴄᴇᴜᴛɪᴄᴀʟ, ᴘʜᴀʀᴍᴀᴄᴇᴜᴛɪᴄᴀʟ, ɴᴀᴛᴜʀᴀʟ ᴘʀᴏᴅᴜᴄᴛ, ᴀɴᴅ ᴇɴᴠɪʀᴏɴᴍᴇɴᴛᴀʟ ᴀɴᴀʟʏsᴇs, ᴀʀᴇ ɪɴᴄʀᴇᴀsɪɴɢʟʏ ʙᴇɪɴɢ ʀᴇᴘᴏʀᴛᴇᴅ.

Answer 2

hello dear here is ur answer

The Ascentis Express F5 HPLC columns have a unique mix of shape selectivity, ionic character, and hydrophobic retention to provide the selectivity needed for the separation of closely related compounds.

Ascentis Express is a high-speed, HPLC column based on Fused-Core particle technology.