For the strand separation and stabilisation during DNA replication which of the following set of enzymes and proteins are required? (1)SSBP,gyrase,primase (2)Topoisomerase,helicase,ligase (3)Gyrase,ligase,primase 4)Topoisomerase,helicase,SSBP
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Answer:
2. is the correct answer
topoisomerase
helicase
ligase
Explanation:
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For strand separation and stabilization during DNA replication, the set of enzymes and proteins required are topoisomerase, helicase, and SSBP.
- DNA replicates by the semi-conservative method. A new complementary strand forms for each DNA strand.
- The steps involved in replication are the Unwinding of DNA, elongation, and ligation of the DNA.
- The DNA unwinds by the topoisomerase.
- And supercoiling of DNA is prevented by the helicase enzyme is used.
- The single-stranded DNA is unstable, so SSBP (Single-Stranded Binding Protein) gets attached to the DNA strand to make it stable.
- After this, DNA polymerase uses in the elongation step.
- And the DNA fragments are joined by the DNA ligase enzyme.
- Hence, the correct answer is an option (4).
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