Formula to calculate bond length without bond length chart
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The length of the bond is determined by the number of bonded electrons (the bond order). The higher the bond order, the stronger the pull between the two atoms and the shorter the bond length. Generally, the length of the bond between two atoms is approximately the sum of the covalent radii of the two atoms.
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Explanation:
When you use a pH lower than the pI then your peptide is most likely positively charged. Higher pH than the pI means negative net charge. Hope I could help with that
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