Question

Formula to calculate catalase activity 240 nm biological samples

Answer 1

Answer:

Catalase unit definition according to Aebi 1974 is: one unit is equivalent to 0.01 decrease in absorbance at 240 nm/mg protein/min.

What you do is to react a known amount of protein extract (say 5 mg protein, which should be calculated based on the protein content of you extract to find out how much volume of sample to use) with a known concentration of peroxide. Once you add all reaction components you read the initial absorbance at time zero. You then wait for a given time (30 s) and then read the absorbance again and record the decrease in absorbance.

To do the calculations:

(Decrease in absorbance x 100/1 ) divided by protein amount in mg divided by time in min=units/mg protein/min.

I hope this helps.