Question

freezing and thawing of cells procedure​

Answer 1

Answer:

Remove the cryovial containing the frozen cells from liquid nitrogen storage and immediately place it into a 37°C water bath. Quickly thaw the cells (< 1 minute) by gently swirling the vial in the 37°C water bath until there is just a small bit of ice left in the vial. Transfer the vial it into a laminar flow hood.

Explanation:

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Answer 2

Answer:

Thawing Method

• Remove the frozen vial from the liquid N2 storage.
• Loosen the cap of the vial slightly to release the pressure inside.
• Thaw the cells in a 37ºC water bath.
• Keep the lid of the freezing vial above the surface of the water to lessen the chances of contamination