Question

From the Lineweaver-Burk plot of Michaelis-Menten equation, Km and Vmax can be determined when V is the reaction velocity at substrate concentration S, the X-axis experimental data are expressed as


Select one:
a. S
b. 1/V
c. V
d. 1/S​

Answer 1

Answer:

V answeransweransweransweransweransweransweranswer

Answer 2

Lineweaver-Burk plot

Explanation:

• We know that the Michaelis-Menten equation can be expressed as$V = \dfrac{V_{max} \times [S]}{K_{m} + [S] }$

• On rearrangement of the above equation, for the convenient graphical form, we get the Lineweaver-Burk plot equation.

         $\dfrac{1}{V} = \dfrac{K_{m}}{V_{max} \times [S] } +\dfrac{1}{V_{max}}$

• On comparing the above equation with the straight-line equation, y=mx + c
• We conclude that 0n y-axis we have 1/V and on X-axis we have 1/[S]
• Thus, the correct option is (d) 1/S