Question

Fundamental difference between adsorption and partition chromatography

Answer 1

Here the molecules get preferential separation in between two phases. i.e., both stationary phase and mobile phase are liquid in nature. So molecules get dispersed into either phases preferentially. Polar molecules get partitioned into polar phase and vice-verse. This mode of partition chromatography applies to Liquid-liquid, liquid-gas chromatography and not to solid-gas chromatography. Because partition is the phenomenon in between a liquid and liquid or liquid and gas or gas and gas. But not in solid involvement.

In high performance liquid chromatography (HPLC), Paper chromatography, gas chromatography, high performance thin layer chromatography (HPTLC), partition chromatography is the principle of separation. While in column chromatography, Thin layer chromatography