G-protein coupled receptors (gpcr) consist of three protein subunits , β and γ. in unstimulated state, subunit is gdp bound and gpcr is inactive. when gpcr gets activated, it acts like guanine nucleotide exchange (gef) factor and induces -subunit to release its bound gdp allowing gtp to bind in its place. in order to regulate g- protein activity by regulating gdp/gtp concentration, subunit acts as
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