Question

gel chromatography instrumentation​

Answer 1

Answer:

1) Gel permeation chromatography is also called as gel filtration or size exclusion chromatography.

2) In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc.

3) The separation is based on the analyte molecular sizes since the gel behaves like a molecular sieve.

4) This technique is used for the separation of proteins, polysaccharides, enzymes, and synthetic polymers.

5) As a technique, size exclusion chromatography was first developed in 1955 by Lathe and Ruthven.

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Answer 2

Is based on the molecular size and the hydrodynamic volume of the components.