Biology, asked by yashaswini20, 1 year ago

gel electrophoresis is considered as a very important technique in recombinant DNA technology why

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Answered by parirajput
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Gel electrophoresis is used for separation and isolation idle DNA fragments. It is a technique used for separation for substances of different ionic properties on electric field DNA fragments are - ive charged molecules moves towards anode according to their molecular size through agrose gel the separated DNA fragments are observed with ethidium bromide solution . the bands of DNA can be seen under UV rays .These bands are cuts from gel and purified.

The DNA bands we got by gel electrophoresis can be used in constructing recombinant DMA by joining them with cloning vector.

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