Question

Gene gun is used in which of the following work​

Answer 1

Gene gun is used to deliver exogenous DNA or RNA or protein to a cell.

• This is a biotechnological tool.
• In this method, the DNA is first coated with gold nano particles, and then it is put to the gun like device and then a force is given to project to the targeted cell.

Answer 2

The correct answer is:

Explanation:

• Gene gun is an in-vitro biotechnological tool.
• It is used to introduce foreign gene, DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) into cells.
• This is done using heavy metals like gold or tungsten.
• The heavy metals are coated with the bio-molecule (DNA or RNA) to be introduced into the cells to form the micro-projectile.
• These micro-projectiles are targeted into the cells by means of mechanical force.
• Inside the cells the foreign material is expected to get integrated into the genome.
• The success of integration is checked using reporter assays, in which a reporter gene that would indicate successful integration is included along with the target gene.
• Positive results of assays checking for presence of reporter gene indicate successful integration of the target gene in the genome.