Question

General methods of isolation and separation of plant constituents

Answer 1

Answer:

Explanation:

It is a common practice in isolation of these bioactive compounds that a number of different separation techniques such as TLC, column chromatography, flash chromatography, Sephadex chromatography and HPLC, should be used to obtain pure compounds.

Answer 2

Isolation and Plant Constituents

Explanation:

• It has been occurs that ethylene glycol monoethyl ether (Cellosolve) will separate all plant constituents that are normally removed by the utilization of petroleum ether, ether, chloroform, and got dried out liquor utilized in a specific order
• A plan of isolating plant constituents is given including their extraction Cello‐solve and fraction division by the expansion of water
• Separate the plant material beginning with hexane (or oil ether), and follow that with solvents of expanding extremity (ethyl acetic acid derivation, ethanol, and water, for instance)
• These concentrates are then exposed to segment chromatography