Genome wide profiling of vertebrate gaf/thpok binding sites reveal its association with lamina associated domains
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Abstract
In metazoan cell nuclei, hundreds of large chromatin domains are in close contact with the nuclear lamina. Such lamina-associated domains (LADs) are thought to help organize chromosomes inside the nucleus and have been associated with gene repression. Here, we discuss the properties of LADs, the molecular mechanisms that determine their association with the nuclear lamina, their dynamic links with other nuclear compartments, and their proposed roles in gene regulation.
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Introduction: the nuclear lamina and genome organization
In metazoan cell nuclei the inner nuclear membrane is lined by the nuclear lamina (NL), a fibrous layer consisting primarily of type V intermediate filament proteins named lamins. Early electron microscopy revealed the tight apposition of a layer of condensed chromatin adjacent to the NL (Fawcett, 1966) (Figure 1A). Later, DNA fluorescence in situ hybridization (FISH) demonstrated that specific genomic loci are preferentially located at the nuclear periphery, often when these loci exhibit low transcriptional activity (reviewed in Lanctot et al., 2007). During the past decade, genome-wide mapping methods have identified genomic regions that are in close contact with the NL, termed Lamina-Associated Domains (LADs).
In metazoan cell nuclei, hundreds of large chromatin domains are in close contact with the nuclear lamina. Such lamina-associated domains (LADs) are thought to help organize chromosomes inside the nucleus and have been associated with gene repression. Here, we discuss the properties of LADs, the molecular mechanisms that determine their association with the nuclear lamina, their dynamic links with other nuclear compartments, and their proposed roles in gene regulation.
Go to:
Introduction: the nuclear lamina and genome organization
In metazoan cell nuclei the inner nuclear membrane is lined by the nuclear lamina (NL), a fibrous layer consisting primarily of type V intermediate filament proteins named lamins. Early electron microscopy revealed the tight apposition of a layer of condensed chromatin adjacent to the NL (Fawcett, 1966) (Figure 1A). Later, DNA fluorescence in situ hybridization (FISH) demonstrated that specific genomic loci are preferentially located at the nuclear periphery, often when these loci exhibit low transcriptional activity (reviewed in Lanctot et al., 2007). During the past decade, genome-wide mapping methods have identified genomic regions that are in close contact with the NL, termed Lamina-Associated Domains (LADs).
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