Question

genomic DNA (2x10⁶ copy) is amplified for uning 30 cycles using a forward and reverse primer. Estimate the no. of target DNA flanked by. by f/R primers, if the PER efficiency is 90%.​

Answer 1

Explanation:

After 30 cycles, what began as a single molecule of DNA has been amplified into more than a billion copies (230 = 1.02 x 109). With PCR, it is routinely possible to amplify enough DNA from a single hair follicle for DNA typing. Some workers have successfully amplified DNA from a single sperm cell.