Question

give a brief account of genetic engineered insulin....

Answer 1

Restriction enzyme = cutting enzyme
Ligase enzyme = sticking enzyme

The restriction enzyme is used to cut the gene that codes for insulin production out of a strand of human DNA. It is also used to remove and cut open a plasmid (loop of DNA) from a bacterium. The ligase enzyme is then used to stick the insulin gene into the plasmid, and then to stick the plasmid loop back together, and insert it back into the bacterium. The bacterium reproduces many times, also reproducing the genetically engineered plasmid. The insulin gene is finally switched on and harvested.

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