Question

Give a brief account of the role of various components of a standard plant tissue culture medium on plant growth in vitro.

Answer 1

The composition of the culture media is primarily dependent on two parameters:

1. The particular species of the plant.

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2. The type of material used for culture i.e. cells, tissues, organs, protoplasts.

Thus, the composition of a medium is formulated considering the specific requirements of a given culture system. The media used may be solid (solid medium) or liquid (liquid medium) in nature. The selection of solid or liquid medium is dependent on the better response of a culture.

Answer 2

Growth and morphogenesis of plant tissue cultures can be improved by small amounts of some organic nutrients. These are mainly vitamins (including some substances that are not strictly animal vitamins), amino acids and certain undefined supplements. The amount of these substances required for successful culture varies with the species and genotype, and is probably a reflection of the synthetic capacity of the explant.

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