. Give a reason why each of the following steps are followed when preparing cross sections
of a leaf for examination under a microscope:
a)Cutting very thin sections
b)Using sharp razor blade (scalpel) during cutting.
c)Placing the sections in water
d)Staining the sections with iodine before observing under a microscope.
Answers
hope it will help you dude....
Answer:
1 A specimen being viewed under a microscope should be thin so that light can pass, Otherwise the light cannot get through the specimen. ... If they were not thin enough for light to pass through them, they would not be visible under a compound microscope.
2 we use razor sharp blade so that we get a clean and proper cut which helps in making examination of cells easy and helps in passing of light in the specimen.
3 we stain them so that the cells are easily visible due to colour or if we use better english:
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope.
Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
we use water because 1) it protects the microscope's objective lens from contacting the specimen, and (2) it creates an even thickness (in wet mounts) for viewing.