Question

Give an account of the process or recombinant DNA technology

Answer 1

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♦ ♦ ♦ Process of Recombinant DNA Technology  ♦ ♦ ♦

Recombinant DNA technology involves the selection of the desired gene for administration into the host followed by a selection of the perfect vector with which the gene has to be integrated and recombinant DNA formed. This recombinant DNA, then has to be introduced into the host. And at last, it has to be maintained in the host and carried forward to the offsprings.

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Answer 2

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Following are the key tools for recombinant DNA technique —

• Enzyme
• Vector DNA
• Passenger DNA

a) Enzymes :- important enzymes are lysozyme, exo - and endonuclease, reverse transcriptase and ligase.

b) vector DNA :- the DNA which by parallel any part of a foreign DNA introduces into favourable host. It is also called gene carrier.

Important example are — plasmid DNA and transposon

C) passenger DNA — the DNA which are carrier from one organism to another through the help of vehicle DNA.